Empirical estimation of free testosterone from testosterone and sex hormone-binding globulin immunoassays
نویسندگان
چکیده
منابع مشابه
Empirical estimation of free testosterone from testosterone and sex hormone-binding globulin immunoassays.
BACKGROUND The growing interest in measuring blood free testosterone (FT) is constrained by the unsuitability of the laborious reference methods for wider adoption in routine diagnostic laboratories. Various alternative derived testosterone measures have been proposed to estimate FT from either additional assay steps or calculations using total testosterone (TT) and sex hormone-binding globulin...
متن کاملTestosterone, sex hormone-binding globulin, calculated free testosterone, and oestradiol in male vegans and omnivores.
Total testosterone (T), total oestradiol (E2) and sex hormone-binding globulin (SHBG) concentrations were measured in plasma samples from fifty-one male vegans and fifty-seven omnivores of similar age. Free T concentration was estimated by calculation. In comparison with the omnivores, the vegans had 7% higher total T (P = 0.250), 23% higher SHBG (P = 0.001), 3% lower free T (P = 0.580), and 11...
متن کاملInvestigation of Sex-Hormone Binding Globulin for Testosterone and Estradiol
The aim of this study was to find reasons for the interference of sex-hormone binding globulin (SHBG) in direct radloimmunoassays (RIAs) for testosterone and estradiol. 1251.. labeled testosterone and estradiol were compared with 3Hlabeled testosterone and estradiol for their affinity to SHBG. The 3H-Iabeled steroids bound far more avidly to SHBG than did the corresponding 1251-labeled steroids...
متن کاملThe Evaluation of Serum Level of Testosterone and Sex Hormone- Binding Globulin in Men with Type 2 Diabetes
OBJECTIVE: To evaluate the relationship between serum level of testosterone and sex hormone-binding globulin in men with type 2 diabetes. MATERIALS AND METHODS: In this case-control study, forty men aged 40-70 with type 2 diabetes mellitus were randomly selected and compared with 40 non-diabetic men. The two groups were matched for their age and BMI. After complete observation and examination...
متن کاملSimultaneous determination of free testosterone and testosterone bound to non-sex-hormone-binding globulin by equilibrium dialysis.
We describe a procedure based on equilibrium dialysis that allows the simultaneous determination of free testosterone and testosterone bound to non-sex-hormone-binding globulin (non-SHBG) in plasma. After saturating SHBG with 5 alpha-dihydrotestosterone (DHT) according to a technique recently described, the percentage of free testosterone in the treated and the untreated samples is measured by ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Endocrinology
سال: 2005
ISSN: 0804-4643,1479-683X
DOI: 10.1530/eje.1.01844